100%

100% route begins at the start of the game, and it ends when the mask shatters at the end of the game. However, the player must also collect all items in the game, including the shop upgrades and 45 Power Seals, before they complete the game.

The inventory menu screen will look like this. The last item slot is normally empty. The Power Thistle will be missing on versions earlier than Version 1.0.4.

Ninja Village

 * Ninja Village.

Autumn Hills

 * Autumn Hills.
 * Autumn Hills Power Seal #1, Cloudstep off of a Fire Kappa fireball to reach this room. | Power Seal Count: 1

Forlorn Temple

 * Forlorn Temple.
 * Go to the room above the entrance of Forlorn Temple, collect the Big Time Shard.

Catacombs

 * Catacombs.
 * Go to the room above the first room of Catacombs, collect the Big Time Shard.
 * Catacombs Power Seal #1, left side of falling section. | Power Seal Count: 2

Bamboo Creek

 * Bamboo Creek.

Howling Grotto

 * Howling Grotto.
 * Howling Grotto Power Seal #1, from 3 Fire Kappa right side. | Power Seal Count: 3

Quillshroom Marsh

 * Quillshroom Marsh.

Searing Crags

 * Searing Crags.
 * Searing Crags Power Seal #1, after climb. | Power Seal Count: 4

Glacial Peak

 * Glacial Peak.

Tower of Time

 * Tower of Time.
 * Tower of Time Power Seal #1, grab the checkpoint after crushers, then backtrack to the previous room for the Power Seal, then die to death-warp. | Power Seal Count: 5
 * Tower of Time Power Seal #2, after dropping through the lasers, attack breakable blocks to reach the Power Seal room. | Power Seal Count: 6
 * Tower of Time Power Seal #4, on the left side after outside parkour. | Power Seal Count: 7

Cloud Ruins

 * Cloud Ruins.

Underworld

 * Underworld.
 * Underworld Power Seal #1, from lava tide room after huge climb. | Power Seal Count: 8

Metroidvania

 * Autumn Hills portal.
 * Autumn Hills Music Note (Key of Hope).
 * Autumn Hills Power Seal #2, be 16-bit while traveling westward to the first shop room. | Power Seal Count: 9
 * Ninja Village.
 * Talk to Ninja Elder for Astral Seed.
 * Ninja Village Power Seal #1, be 16-bit. | Power Seal Count: 10
 * Autumn Hills portal.
 * Catacombs shortcut.
 * Dark Cave.
 * Rivière Turquoise.
 * Rivière Turquoise Power Seal #1, right side of waterfall after portal. | Power Seal Count: 11
 * Rivière Turquoise Power Seal #2, fall down left waterfall after first fairy lights. | Power Seal Count: 12
 * Rivière Turquoise Power Seal #3, left side of Blue Kappa room breakable rock after turning to 16-bit. | Power Seal Count: 13
 * Defeat Butterfly Matriarch.
 * Autumn Hills portal.
 * Catacombs shortcut.
 * Catacombs Power Seal #2, above Dark Cave entrance. | Power Seal Count: 14
 * Catacombs Power Seal #3, two rooms to the right. | Power Seal Count: 15
 * Be 16-bit, grab Ruxxtin's Amulet from the boss room.
 * Bamboo Creek.
 * Bamboo Creek Power Seal #1, from left of the first shop. | Power Seal Count: 16
 * Bamboo Creek Power Seal #2, from under the elevator room. | Power Seal Count: 17
 * Bamboo Creek Power Seal #3, from diagonal elevator right side. | Power Seal Count: 18
 * Grab Claustro the Phobekin.
 * Howling Grotto.
 * Take underwater maze.
 * Sunken Shrine.
 * Sunken Shrine Power Seal #1, above 16-bit room before the Lightfoot Tabi. | Power Seal Count: 19
 * Grab Lightfoot Tabi.
 * Sunken Shrine Power Seal #2, from right side at bottom-right of first descent. | Power Seal Count: 20
 * Grab Moon Crest.
 * Sunken Shrine Power Seal #3, from left side of first descent room. | Power Seal Count: 21
 * Grab Sun Crest.
 * Sunken Shrine Music Note (Key of Love).
 * Go right, collect the Big Time Shard.
 * Go left, teleport to Tower of Time HQ.
 * Howling Grotto portal.
 * Howling Grotto Power Seal #2, backtrack to Power Seal in time-shifting room. | Power Seal Count: 22
 * Howling Grotto Power Seal #3, in updraft room, switch to 16-bit, then fall back down to go to Power Seal room. | Power Seal Count: 23
 * Howling Grotto 2 (???)
 * Quillshroom Marsh.
 * Grab Magic Seashell.
 * Quillshroom Marsh Power Seal #1, above room after Magic Seashell. | Power Seal Count: 24
 * Quillshroom Marsh Power Seal #2, room below the second shop. | Power Seal Count: 25
 * Quillshroom Marsh Power Seal #3, switch to 8-bit before this Power Seal room. | Power Seal Count: 26
 * 8-bit, interact with Queen of Quills.
 * Switch to 16-bit, go to Searing Crags.
 * Searing Crags Power Seal #2, left side of first climb. | Power Seal Count: 27
 * Searing Crags Power Seal #3, left side of final climb. | Power Seal Count: 28
 * 16-bit, grab Power Thistle.
 * Unlock Searing Crags portal.
 * 8-bit, give Power Thistle.
 * Searing Crags Music Note (Key of Strength).
 * Searing Crags portal.
 * Grab Pyro the Phobekin.
 * Underworld Power Seal #2, in the room after the lava rise. | Power Seal Count: 29
 * Underworld Power Seal #3 and #4, after the climb. | Power Seal Count: 30, 31
 * Underworld Music Note (Key of Chaos).
 * Glacial Peak portal.
 * Glacial Peak Power Seal #1, after triple-lantern descent. | Power Seal Count: 32
 * 8-bit, talk to Monk so she breaks Manfred's lanterns.
 * Glacial Peak Power Seal #2, down to the bottom of the mountain in 16-bit. | Power Seal Count: 33
 * Glacial Peak Power Seal #3, climb north to reach middle Power Seal room. | Power Seal Count: 34
 * Climb north, free Manfred.
 * Elemental Skylands.
 * Air Skyland.
 * Elemental Skylands Power Seal #1, on the left side after the second climb. | Power Seal Count: 35
 * Earth Skyland.
 * Water Skyland.
 * Elemental Skylands Power Seal #2, on the right side in the second room. | Power Seal Count: 36
 * Fire Skyland.
 * Elemental Skylands Power Seal #3, under the island when crossing underneath. | Power Seal Count: 37
 * Defeat Clockwork Concierge, obtain Elemental Skylands Music Note (Key of Symbiosis).
 * Glacial Peak portal.
 * Cloud Ruins.
 * Cloud Ruins Power Seal #1, climb after spike wall jumps. | Power Seal Count: 38
 * Cloud Ruins Power Seal #2, after descent after returning to 16-bit, on the left. | Power Seal Count: 39
 * Cloud Ruins Power Seal #3, bottom-left of the updraft room with many Time Shards. | Power Seal Count: 40
 * Grab Acro the Phobekin, but don't death-warp.
 * Cloud Ruins Power Seal #4, go to the room above the last shop of Cloud Ruins. | Power Seal Count: 41
 * Autumn Hills portal.
 * Return to Ninja Village, talk to Ninja Elder for Ordinary Wax Candle.
 * Autumn Hills portal.
 * Autumn Hills 2 (???)
 * Forlorn Temple, switch to 8-bit.
 * Autumn Hills Power Seal #3 and #4, below the ground after descent. | Power Seal Count: 42, 43
 * Forlorn Temple.
 * Forlorn Temple Power Seal #1, after a long room with platforms, change to 16-bit in the next room then return back to the long room. | Power Seal Count: 44
 * Forlorn Temple Power Seal #2, after 3-rocket room, go up to Power Seal room. | Power Seal Count: 45
 * Defeat Demon King, obtain Demon Crown.
 * Corrupted Future.
 * Corrupted Future Music Note (Key of Courage).
 * Music Box.
 * Dunzo.